[1]贯春雨,陈肃,邢政华,等. 11个杨树品种SSR指纹图谱构建及遗传关系研究[J].西北林学院学报,2023,38(2):107-112.[doi:10.3969/j.issn.1001-7461.2023.02.14]
　GUAN Chun-yu,CHEN Su,XING Zheng-hua,et al. Fingerprint and Genetic Analysis of 11 Varieties of Populus Using SSR Markers[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2023,38(2):107-112.[doi:10.3969/j.issn.1001-7461.2023.02.14]
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 11个杨树品种SSR指纹图谱构建及遗传关系研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第38卷
期数:
2023年第2期
页码:
107-112
栏目:
出版日期:
2023-03-31

文章信息/Info

Title:
 Fingerprint and Genetic Analysis of 11 Varieties of Populus Using SSR Markers
文章编号:
1001-7461(2023)02-0107-06
作者:
 贯春雨1; 陈肃2; 邢政华3; 李树森3; 王福森3*
 （1.齐齐哈尔大学，黑龙江 齐齐哈尔 161001；2.东北林业大学 林木遗传育种与生物技术教育部重点实验室，黑龙江 哈尔滨 150040；3.黑龙江省林业科学院齐齐哈尔分院，黑龙江 齐齐哈尔 161005）
Author(s):
 GUAN Chun-yu1; CHEN Su2; XING Zheng-hua3; LI Shu-sen3; WANG Fu-sen3*
 （1.Qiqihar University,Qiqihar 161006,Heilongjiang,China; 2.Northeast Forestry University,State Key Laboratory of Tree Genetic Breeding,Harbin 150040,Heilongjiang,China; 3.Qiqihar Branch of the Heilongjiang Academy of Forestry Sciences,Qiqihar 161005,Heilongjiang,China）
关键词:
 ; 杨树; 分子标记; SSR; 遗传多样性; 聚类分析
Keywords:
Populus;  molesular marker;  SSR ;  genetic diversity;  cluster analysis
分类号:
S722.3
DOI:
10.3969/j.issn.1001-7461.2023.02.14
文献标志码:
A
摘要:
 利用杨树基因组序列设计的SSR引物对11份杨树品种材料进行遗传多样性分析，并构建指纹图谱。结果表明，共27对引物具有多态性，引物多态性比率占69.23%；获得多态性谱带38个，多态性比率为84.4%。SSR遗传多样性分析结果为，有效等位基因数平均值1.578 9，基因多样性指数平均值为0.321 7，Shannon多样性指数平均值为0.471 4。11份杨树品种的遗传距离0.53～0.93，平均值为0.73。聚类分析表明，在遗传距离 0.76处可将所有供试材料分为5 个类群，分别包含2、4、2、1、2份供试材料。研究表明，供试材料遗传多样性较高；利用SSR标记构建了不同杨树利用的指纹图谱，可用于杨树品种间的鉴定。研究结果可为杨树品种知识产权保护和杂交育种提供理论依据。
Abstract:
 Primers were designed according to poplar genome sequence.The fingerprints and genetic diversity of 11 Populus varieties were analyzed by SSR molecular markers.By 38 pairs of primers,27 pairs of primers were polymorphic，the ratio of polymorphic primers accounted for 69.23%.And 38 alleles were amplified.The polymorphism ratio was 84.44%.Genetic diversity analysis showed that the mean effective number of alleles（NE）was 1.578 9，the mean Nei’s gene diversity (H) was 0.321 7,and the mean Shannon’s information index (I) was 0.471 4.The genetic distance of the 11 Populus varieties ranged from 0.53 to 0.93 with an average of 0.73.Cluster analysis showed that all the test materials could be divided into five groups,with the genetic distance 0.76,and the included numbers of variety were 2,4,2,1 and 2,respectively.The results indicated that the test materials had a broad genetic basis,and the genetic diversity of them was high.The fingerprints of each Populus variety were constructed according to SSR markers,which could be used for the identification among Populus varieties,and to provide theoretical basis for intellectual property protection and cross breeding of Populus varieties.

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备注/Memo

备注/Memo:
 收稿日期：2022-03-23修回日期：2022-05-15
基金项目：黑龙江省省属高等学校基本科研业务费青年创新人才项目（135409218）。
第一作者：贯春雨，博士，副教授。研究方向：林木遗传育种。E-mail:gcy19810201@163.com
*通信作者：王福森，硕士，研究员。研究方向：林木遗传育种。E-mail:wangfs66@163.com
更新日期/Last Update: