[1]孙甜甜,任强,陈文义,等. 杨树水通道蛋白基因PagPIP1;3耐干旱胁迫功能分析[J].西北林学院学报,2023,38(4):11-17.[doi:10.3969/j.issn.1001-7461.2023.04.02]
　SUN Tian-tian,REN Qiang,CHEN Wen-yi,et al. Functional Analysis of Poplar Aquaporin Gene PagPIP1;3 Under Drought Stress[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2023,38(4):11-17.[doi:10.3969/j.issn.1001-7461.2023.04.02]
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 杨树水通道蛋白基因PagPIP1;3耐干旱胁迫功能分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第38卷
期数:
2023年第4期
页码:
11-17
栏目:
出版日期:
2023-07-31

文章信息/Info

Title:
 Functional Analysis of Poplar Aquaporin Gene PagPIP1;3 Under Drought Stress
文章编号:
1001-7461(2023)04-0011-07
作者:
 孙甜甜1; 任强2; 陈文义1; 衡启蒙1; 温昱斌3; 任若寒1; 李想1; 张银燕1; 张丽娟1; 贺浩冉1; 樊军锋1; 张一1*
 （1.西北农林科技大学 林学院，国家林业和草原局黄土高原林木培育重点实验室，陕西 杨陵 712100；2.陕西省延安市宝塔区姚家坡国有生态林场，陕西 延安 716000；3.赣州市天力能源科技咨询服务有限公司，江西 赣州 341000）
Author(s):
 SUN Tian-tian1; REN Qiang2; CHEN Wen-yi1; HENG Qi-meng1; WEN Yu-bin3; REN Ruo-han1; LI Xiang1; ZHANG Yin-yan1; ZHANG Li-juan1; HE Hao-ran1; FAN Jun-feng1; ZHANG Yi1*
 (1.College of Forestry/Jujube Engineering Technology Center,National Forestry and Grassland Administration,Northwest A&F University,Yangling 712100,Shaanxi,China; 2.Yaojiapo State-Owned Ecological Forest Farm in Baota District,Yan’an 716000,Shaanxi,China;3.Ganzhou Tianli Energy Technology Consulting Service Corporation,Ganzhou 341000,Jiangxi,China)
关键词:
 ; 杨树; 干旱; PagPIP1; 3; 遗传转化; 功能分析
Keywords:
 ; poplar;  drought;  PagPIP1; 3;  transformation;  functional analysis
分类号:
S792.11
DOI:
10.3969/j.issn.1001-7461.2023.04.02
文献标志码:
A
摘要:
 干旱是自然界中最常见的环境胁迫因子之一。杨树主要分布于我国北方半干旱地区，干旱和水分匮乏严重限制了杨树生长，降低了杨树人工林生产力。为阐明杨树耐干旱的分子机制，推动杨树耐旱的分子育种，将前期鉴定出的干旱胁迫响应的关键PIP1s基因PagPIP1;3作为目标基因，通过农杆菌转化法获得PagPIP1;3过表达银中杨阳性株系，从形态和生理方面验证了PagPIP1;3在杨树响应和耐受耐旱胁迫中的功能。结果表明，与野生型对照植株（WT）相比，过表达PagPIP1;3的不同株系（OE1，OE2）分别在不同器官部位出现基因表达量的显著增加，在叶片中基因表达量OE2>OE1>WT；根系中基因表达量OE1>OE2>WT。在干旱胁迫条件下，PagPIP1;3过表达株系地上部分和根系的生长量明显优于WT，并且在一定程度上抑制了银中杨的蒸腾、增强了银中杨在干旱胁迫下的瞬时水分利用效率（WUEi）。对生物量和相对含水量测定结果表明，PagPIP1;3过表达后，水分在干旱胁迫下的转运能力得到提高，杨树叶片保水能力和生物量的积累得到增强，从而提高了杨树的耐旱能力。研究结果初步阐明了PagPIP1;3基因在杨树耐干旱胁迫中的功能及机制，为杨树耐干旱胁迫的分子遗传改良提供了重要研究基础和遗传材料。
Abstract:
 Drought is one of the most common environmental stress factors to plants.It is an urgent need for efficiently breeding drought resistant poplar varieties to break through the limitation of drought.In this study,a candidate poplar gene with drought stress tolerance PagPIP1;3 was used for genetic transformation.The overexpressed lines of PagPIP1;3 were obtained by agrobacterium transformation method,and the function of the overexpressed lines in drought stress was explored by morphological and physiological investigations.The different lines of PagPIP1;3-overexpression showed the advantages in different organs.In leaves,the ranking of expression level was OE2>OE1>WT.In roots,the expression level ranking was OE1>OE2>WT.Under drought stress,the growth of aboveground part and root system of overexpression lines were significantly better than WT,and PagPIP1;3-overexpression inhibited the transpiration rate and enhanced the WUEi of poplar under drought stress.The biomass and relative water content of overexpressed lines also improved,indicating that PagPIP1;3-overexpression improved the drought tolerance of poplar by promoting the ability of water transporting and biomass accumulating under drought stress.These results provide an important basis for clarifying the mechanism of PagPIP1;3-overexpression under drought stress.

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备注/Memo

备注/Memo:
 收稿日期：2022-05-20 修回日期：2022-06-17
基金项目：国家自然科学基金（32171823）。
第一作者：孙甜甜。研究方向：林木遗传育种。E-mail：suntiantian1220@163.com
*通信作者：张一，博士生导师，副研究员。研究方向：林木遗传育种。E-mail:zy780711@163.com
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