[1]刘春英,樊军锋,高建社,等. 杨树新杂种的SSR分析及鉴定[J].西北林学院学报,2013,28(02):70-73.[doi:10.3969/j.issn.1001-7461.2013.02.13]
　LIU Chun-ying,FAN Jun-feng,GAO Jian-she,et al. Simple Sequence Repeat Analysis and Identification of New Populus Hybrid[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(02):70-73.[doi:10.3969/j.issn.1001-7461.2013.02.13]
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 杨树新杂种的SSR分析及鉴定()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第28卷
期数:
2013年02期
页码:
70-73
栏目:
出版日期:
2013-03-30

文章信息/Info

Title:
 Simple Sequence Repeat Analysis and Identification of New Populus Hybrid
文章编号:
1001-7461(2013)02-0070-04
作者:
 刘春英; 樊军锋; 高建社; 周永学; 魏宁
 (西北农林科技大学 林学院， 陕西 杨陵 712100)
Author(s):
 LIU Chun-ying; FAN Jun-feng; GAO Jian-she; ZHOU Yong-xue; WEI Ning
 (College of Forestry,Northwest A＆F University,Yangling,Shaanxi 712100,China)
关键词:
 ; 杨树; 杂交种; SSR; 品种鉴定 ; 指纹图谱
Keywords:
 ; poplars;  hybrid;  SSR;  cultivar identification;  fingerprint
分类号:
S792.110.4
DOI:
10.3969/j.issn.1001-7461.2013.02.13
文献标志码:
A
摘要:
 以2个杨树杂交新品种（编号分别为：02-9-22，02-12-29）及其亲本和毛白杨无性系30号为材料，从36对SSR引物中筛选出在杂交新品种双亲之间多态性明显、重复性比较好的12对引物，用于杨树的遗传变异分析。共扩增出125个DNA片段，其中111个片段呈现多态性，占总扩增片段的88.8%，片段大小介于80~1 000 bp之间。基因型间的平均遗传相似系数为0.445 9。聚类分析结果表明，杂交种与亲本的亲缘关系较近，与毛白杨30号的亲缘关系较远。选用3对引物构建5个杨树品种的指纹图谱，在该图谱中，每个品种都有自身独特的片段。
Abstract:
 Analysis of genetic variations was carried out by using genomic DNA of two new Populus hybrid cultivars (Code:02-9-22 and 02-12-29) and their parents as materials,and simple sequence repeat (SSR) molecular technology was adopted.Populus tomentosa 30 was used as control.Among 36 pairs of SSR primers,12 pairs of primers which presented apparent polymorphism between the parents were determined to analyze genetic diversity for Populus cultivars.Fragments of 125 DNA were amplified,of which 111 were polymorphic (accounting for 88.8% of the total).Fragment size ranged from 80 to 1 000 bp.The average genetic similarity between the genotypes of the cultivars was 0.445 9.Cluster analysis showed that high similarity coefficient existed between new Populus hybrid cultivars and their parents.However,low similarity coefficients were found between the two hybrids and P. tomentosa 30.Three pairs of primers were selected to construct DNA fingerprints of 5 Populus cultivars.In this fingerprint,every cultivar had special fragment.

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备注/Memo

备注/Memo:
 收稿日期：2012-05-20 修回日期：2012-09-12
基金项目：“十二五”农村领域国家科技计划专题；“超高产优质毛白杨新品种选育”（2012BAD01B0302）。
作者简介：刘春英，女，硕士研究生，主要研究方向：林木遗传育种林业生物技术。E-mail:liuchy87@163.com
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