[1]任若寒,王海泽,吴承生,等.杨树脯氨酸合成关键基因PagP5CS1全长克隆及功能验证[J].西北林学院学报,2023,38(4):90-96.[doi:10.3969/j.issn.1001-7461.2023.04.11]
　REN Ruo-han,WANG Hai-ze,WU Cheng-sheng,et al. Full-Length Cloning and Functional Berification of PagP5CS1 From Populus alba×P.glandulosa[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2023,38(4):90-96.[doi:10.3969/j.issn.1001-7461.2023.04.11]
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杨树脯氨酸合成关键基因PagP5CS1全长克隆及功能验证()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第38卷
期数:
2023年第4期
页码:
90-96
栏目:
出版日期:
2023-07-31

文章信息/Info

Title:
 Full-Length Cloning and Functional Berification of PagP5CS1 From Populus alba×P.glandulosa
文章编号:
1001-7461(2023)04-0090-07
作者:
 任若寒1; 王海泽2; 吴承生3; 衡启蒙1; 陈文义1; 孙甜甜1; 张丽娟1; 贺浩冉1; 李想1; 张银燕1; 樊军锋1; 张一1*
 （1.西北农林科技大学 林学院，国家林业和草原局黄土高原林木培育重点实验室，陕西 杨陵 712100；2.陕西省安康市林业技术推广中心，陕西 安康 725000；3.江西省赣州市崇义县农业农村局，江西 赣州 341399）
Author(s):
 REN Ruo-han1; WANG Hai-ze2; WU Cheng-sheng3; HENG Qi-meng1; CHEN Wen-yi1; SUN Tian-tian1; ZHANG Li-juan1; HE Hao-ran1; LI Xiang1; ZHANG Yin-yan1; FAN Jun-feng1; ZHANG Yi1*
 (1.College of Forestry,Key Laboratory of Tree Cultivation on Loess Plateau,National Forestry and Grassland Administration,Northwest A&F University,Yangling 712100,Shaanxi,China; 2.Shaanxi Ankang Forestry Technology Extension Center,Ankang 725000,Shannxi,China; 3.Ganzhou City,Jiangxi Province Chongyi County Bureau of Agriculture and Rural affairs,Ganzhou 341399,Jiangxi,China)
关键词:
 ; 杨树; 脯氨酸; P5CS1; 干旱
Keywords:
 ; poplar;  proline;  P5CS1;  drought
分类号:
S792.11
DOI:
10.3969/j.issn.1001-7461.2023.04.11
文献标志码:
A
摘要:
 杨树是重要的工业用材树种。我国北方干旱和半干旱地区水分匮乏，干旱已成为影响杨树人工林可持续发展的一个重要因素。游离脯氨酸的积累常常在植物应对干旱等渗透胁迫时出现，以缓解干旱胁迫带给植物的损害。P5CS作为编码脯氨酸合成关键酶的基因，对于植物调控游离脯氨酸合成、增强耐干旱能力非常关键。该研究克隆84K杨脯氨酸合成代谢的关键基因PagP5CS1并在银中杨内进行遗传转化，从表型、分子和生理水平上对PagP5CS1转基因杨树株系进行了功能鉴定。本研究构建的PagP5CS1超表达载体通过叶盘法转化至银中杨叶片，成功获得12个PagP5CS1超表达杨树株系，其中P5CS1基因表达量最高的转基因株系为野生型表达量的8.3倍。经过干旱胁迫处理，相对于野生型，超表达株系叶片萎蔫程度更低；在干旱胁迫下，超表达株系中P5CS1基因表达量和脯氨酸含量均高于野生型，说明P5CS1超表达增强了杨树的耐旱性，P5CS1基因在干旱胁迫下发挥了正向调控作用。综上所述，杨树P5CS1基因对干旱胁迫高度响应，PagP5CS1超表达杨树会通过增加脯氨酸积累来增强杨树的耐旱性。
Abstract:
 Poplar (Populus) is an important timber production species for industry.Drought has become an important factor affecting the sustainable development of poplar plantations in arid and semi-arid areas in Northern China.Under osmotic stress,such as drought,plants often accumulate free proline to alleviate the damage caused by stress.P5CS,a gene encoding a key enzyme of proline synthesis,plays an important role in regulating free proline synthesis and enhancing drought tolerance in plants.In this study,PagP5CS1,a key gene of proline biosyhthesis,was cloned from P.alba×P.glandulosa and transformed into P.alba×P.berolinensis,and the function of PagP5CS1 transgenic poplar was identified from phenotypic,molecular and physiological levels.The constructed PagP5CS1 overexpression vector was transformed into P.alba×P.berolinensis by leaf disk method,and 12 poplar lines with PagP5CS1 overexpression were successfully obtained,among which the transgenic line with the highest expression level was 8.3 times that of the wild type.Compared with the wild type,leaf wilting degree of overexpression lines was lower after drought stress treatment.Under drought stress,the gene expression level of P5CS1 and proline content in the overexpression lines were higher than those in the wild type,indicating that the overexpression of P5CS1 enhanced the drought tolerance of poplar,and P5CS1 played a positive regulatory role.In conclusion,P5CS1 of poplar is highly responsive to drought stress,and PagP5CS1 overexpression poplar can enhance drought tolerance by increasing proline accumulation.

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　WANG Meng chang,LIANG Jun*,FAN Jun feng,et al.Field Investigation on Resistance to Canker of Poplar Variety[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(4):122.
[2]刘莉丽,曹支敏,樊军锋,等.杨树品种叶锈病抗病性研究[J].西北林学院学报,2008,23(06):132.
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[3]高建社,刘玉媛,符毓秦,等.不同产地单株美洲黑杨×青杨F1苗期测定研究[J].西北林学院学报,2004,19(01):55.
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　Advances in the Study of Fertilization on Poplar Plantation[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2004,19(4):67.
[5]杨建伟,韩蕊莲,刘淑明,等.不同土壤水分下杨树的蒸腾变化及抗旱适应性研究[J].西北林学院学报,2004,19(03):7.
　Transpiration and Drought Resistance of Poplar under Different Soil Drought[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2004,19(4):7.
[6]陈建珍,曹支敏,樊军锋.杨树叶锈病寄主抗性调查[J].西北林学院学报,2005,20(01):153.
　Investigation of Poplar Resistance to Melampsora larici-populina[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2005,20(4):153.
[7]苏东凯,周永斌*,唐庆华,等.不同杨树品种光合生理生态特性的研究[J].西北林学院学报,2006,21(02):39.
　SU Dongkai,ZHOU Yongbin*,TANG Qinghua,et al.Ecophysiological Characteristis of Photosynthesis in Different Poular Trees[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2006,21(4):39.
[8]李鸣,周永斌*.辽阳地区不同杨树品种光合速率的研究[J].西北林学院学报,2006,21(06):28.
　LI Ming,ZHOU Yongbin.Studies on Photosynthesis of Different Poplar Species in Liaoyang Area[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2006,21(4):28.
[9]王迪海,赵忠,张彦. 黄土高原刺槐细根与土壤水分特征[J].西北林学院学报,2012,27(01):1.
　WANG Di-hai,ZHAO Zhong,ZHANG Yan. The Fine Root of Robinia pseudoacacia and Soil Moisture in the Loess Plateau[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(4):1.
[10]耿生莲. 不同土壤水分下黑果枸杞生理特点分析[J].西北林学院学报,2012,27(01):6.
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[11]费昭雪,陈祖静,曹支敏,等. 叶锈菌对不同杨树品种叶片木质素的影响[J].西北林学院学报,2012,27(01):124.
　FEI Zhao-xue,CHEN Zu-jing,CAO Zhi-min,et al. Effects of Melampsora larici-populina Kleb. on Leaf Lignin of Poplar Varieties with Different Resistance[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(4):124.
[12]刘丹,逯华涛,姜岳忠,等. 几个杨树无性系对天牛抗性的评价[J].西北林学院学报,2012,27(03):72.
　LIU Dan,LU Hua-tao,JIANG Yue-zhong,et al. Evaluation of Resistance to Cerambycidae of Poplar Clones[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(4):72.
[13]刘春英,樊军锋,高建社,等. 杨树新杂种的SSR分析及鉴定[J].西北林学院学报,2013,28(02):70.[doi:10.3969/j.issn.1001-7461.2013.02.13]
　LIU Chun-ying,FAN Jun-feng,GAO Jian-she,et al. Simple Sequence Repeat Analysis and Identification of New Populus Hybrid[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(4):70.[doi:10.3969/j.issn.1001-7461.2013.02.13]
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　GAO Guang-qin,MEN Qing-ling,HUANG Jia-rong*. Prediction of Poplar Protein Secondary Structure with Artificial Neural Networks[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(4):59.[doi:doi:10.3969/j.issn.1001-7461.2014.05.11]
[18]邱兴,樊军锋*,李晓东,等. 6个杨树新无性系叶片旱生结构研究[J].西北林学院学报,2015,30(01):96.[doi:doi:10.3969/j.issn.1001-7461.2015.01.15]
　QIU Xing,FAN Jun-feng*,LI Xiao-dong,et al. Research on Drought Resistances on Leaf Anatomical Structures of Six New Poplar Clones[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(4):96.[doi:doi:10.3969/j.issn.1001-7461.2015.01.15]
[19]樊蓉,樊军锋,李周岐*. 9个白杨品种SSR指纹图谱构建及遗传关系的研究[J].西北林学院学报,2015,30(03):76.[doi:doi:10.3969/j.issn.1001-7461.2015.03.14]
　FAN Rong,FAN Jun-feng,LI Zhou-qi*. Fingerprinting and Genetic Relatedness of 9 Varieties in Populus L. Sect. Populus Using SSR Markers[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(4):76.[doi:doi:10.3969/j.issn.1001-7461.2015.03.14]
[20]冯连荣,宋立志,张妍,等. 根癌农杆菌介导杨树遗传转化的影响因素[J].西北林学院学报,2015,30(03):120.[doi:doi:10.3969/j.issn.1001-7461.2015.03.21]
　FENG Lian-rong,SONG Li-zhi,ZHANG Yan,et al. Influence Factors on Agrobacterium mediated Transformation Efficiency of Populus[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(4):120.[doi:doi:10.3969/j.issn.1001-7461.2015.03.21]

备注/Memo

备注/Memo:
 收稿日期：2022-05-20 修回日期：2022-06-16
基金项目：国家自然科学基金（32171823）。
第一作者：任若寒。研究方向：林木遗传育种。E-mail：ruohan12062021@163.com
*通信作者：张一，博士，副研究员，博士生导师。研究方向：林木遗传育种。E-mail：zhangyi780711@163.com
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