[1]王磊,解孝满,李文清*,等. 山东核桃主要分布区种质资源遗传多样性AFLP分析[J].西北林学院学报,2014,29(03):113-118.[doi:doi:10.3969/j.issn.1001-7461.2014.03.23]
　WANG Lei,XIE Xiao-man,LI Wen-qing*,et al. AFLP Analysis on the Genetic Diversity of Juglans regia Germplasm Resources from Main Distribution Areas in Shandong Province[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(03):113-118.[doi:doi:10.3969/j.issn.1001-7461.2014.03.23]
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 山东核桃主要分布区种质资源遗传多样性AFLP分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年03期
页码:
113-118
栏目:
出版日期:
2014-05-30

文章信息/Info

Title:
 AFLP Analysis on the Genetic Diversity of Juglans regia Germplasm Resources from Main Distribution Areas in Shandong Province
文章编号:
1001-7461(2014)03-0113-06
作者:
 王磊; 解孝满; 李文清*; 韩彪; 刘丹; 韩庆军
 (山东省林木种质资源中心，山东 济南 250014)
Author(s):
 WANG Lei; XIE Xiao-man; LI Wen-qing*; HAN Biao; LIU Dan; HAN Qing-jun
 (Shandong Forest Germplasm Resources Center,Jinan.Shandong 250014, China)
关键词:
 ; 核桃;  种质资源;  遗传多样性;  AFLP;  聚类分析;  主坐标分析
Keywords:
Juglans regia;  germplasm resource;  genetic diversity;  AFLP;  cluster analysis;  principal coordinate analysis
分类号:
S759.3
DOI:
doi:10.3969/j.issn.1001-7461.2014.03.23
文献标志码:
A
摘要:
 利用AFLP技术对山东省核桃主要分布区收集的55份核桃材料进行遗传多样性分析。结果表明，9对AFLP引物共扩增出566条谱带，其中440条为多态性条带，占总条带数的77.82%。聚类分析表明，材料间的遗传相似系数变幅为0.67～0.86；位点多态性信息含量(PIC)变幅为0.705～0.904，平均值为0.786；在遗传相似系数0.738水平上，55份核桃种质资源可聚成3大类。AFLP标记揭示出该55份核桃各群体之间存在较大遗传变异，各地区群体内遗传相似系数较高。
Abstract:
 Genetic diversity of 55 walnut(Juglans regia)germplasms collected in Shandong were analyzed by AFLP technique.Total 566 bands were amplified from 9 primer combinations, and 440 bands（77.82%）displayed polymorphism.Cluster analysis indicated that the variance range of genetic similarity coefficient of AFLP markers among the germplasm resources were 0.67-0.86;the polymorphic information content (PIC) was 0.705-0.904,averaged 0.786;a dendrogram was constructed using UPGMA based on genetic distance matrix.Three clusters were defined at the genetic similarity of 0.738,AFLP markers proclaimed hereditary difference existed among 55 walnuts germplasm resources and high polymorphism existed in population.

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备注/Memo

备注/Memo:
 收稿日期：2013-06-29 修回日期：2013-12-10
基金项目：山东省农业良种工程重大课题(鲁农良字[2010]6号)。
作者简介：王磊，男，工程师，硕士,研究方向：林木种质资源收集与保护。E-mail：wylm_1203@sina.com
*通信作者：李文清，男，高级工程师，硕士，研究方向：林木种质资源保护与利用。E-mail：xxm529@126.com
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